E-MTAB-1114 - MicroRNA profiling by array of mouse skeletal muscle cells treated with TNF-alpha or IGF-I
Released on 15 May 2013, last updated on 3 May 2014
Murine skeletal muscle cells pmi28 were cultured in growth medium (Ham’s F10, supplemented with 20 % FCS, 2 mM L-glutamine, and 1 % Penicillin / Streptomycin). Myoblasts were cultured on laminin-1 coated dishes for an additional 24 h before switching a fraction of dishes to differentiation medium (DMEM medium containing 2 % horse serum, 2 mM L glutamine, and 1 % Penicillin / Streptomycin) and to differentiation medium containing either 2 x 103 U/ml mouse recombinant TNF-? (Roche Applied Science), or 5 µg/ml mouse recombinant IGF-I (Sigma-Aldrich), or carrier only. pmi28 cells (TNF-? and IGF-I treatments as well as controls) were harvested 24 h after the induction of differentiation. The experiments were performed in quadruplicates or triplicates, respectively.
microRNA profiling by array, co-expression, development or differentiation, in vitro
Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study. Meyer SU, Kaiser S, Wagner C, Thirion C, Pfaffl MW. PLoS One 7(6):e38946 (2012)