6 protocols
AccessionNameType
P-MTAB-26619
bioassay_data_transformation
The array image data (.CEL files) is processed by Model-based analysis of Tiling-array (MAT), to generate a binary file (.bar) that descrive MATscores of every probe on the array. Genomic location is derived from UCSC database (mm9). The resultant .bar file is converted into the txt file.
P-MTAB-26614
nucleic_acid_extraction
P-TABM-3006
P-MTAB-26615
PCR_amplification
Genomic DNA digested by a methylation sensitive restriction enzyme, HpyCH4IV, are amplified by ligation mediated PCR.
P-MTAB-26616
labeling
P-TABM-5265
P-MTAB-26617
hybridization
Hybridization is carried-out using Hybridization oven 645 (Affymetrix) and GeneChip Fluidics Station 450 (Affymetrix) by according to the manufacture's instruction.
P-MTAB-26618
image_acquisition
Microarray is sccaned using GeneChip 3000 7G Scanner with GCOS (v1.4) (Affymetrix)