E-MTAB-1096 - Three distinct patterns of histone H3Y41 phosphorylation mark active genes

Released on 15 April 2013, last updated on 3 June 2014
Homo sapiens
Samples (7)
Protocols (6)
The aim of the project was to identify the global distribution of H3Y41ph and correlate its presence with the transcription factor STAT5 and the active histone modification H3K4me3. This was done in human erythroid leukaemia cells (HEL). HEL cells were treated with DMSO or a JAK2 inhibitor (TG101209) for 4 hours they were then crosslinked with formaldehyde and Chromatin immunoprecipitation was performed using antibodies against the proteins of interest. The DNA was then sequenced on the solexa platform. The analysis includes 7 samples: (1) H3Y41ph with DMSO, (2) H3Y41ph with TG101209, (3) STAT5 with DMSO, (4) STAT5 with TG101209, (5) IgG with DMSO, (6) H3K4me3 with DMSO, (7) independent replicate with H3Y41ph in DMSO
Experiment types
ChIP-seq, binding site identification, high throughput sequencing
Three distinct patterns of histone H3Y41 phosphorylation mark active genes. Dawson MA, Foster SD, Bannister AJ, Robson SC, Hannah R, Wang X, Xhemalce B, Wood AD, Green AR, Göttgens B, Kouzarides T. Cell Rep. 2(3):470-7 (2012), Europe PMC 22999934
Exp. designProtocolsFactorsProcessedSeq. reads