11 protocols
AccessionNameType
P-MTAB-26187
bioassay_data_transformation
Summarisation of the calls in GenomeStudio version 1.9.0.24624. Background corrected, quantile normalised.
P-MTAB-26186
bioassay_data_transformation
Summarisation of the calls in GenomeStudio version 1.9.0.24624. No normalisation, no background correction.
P-MTAB-26185
image_acquisition
Arrays were scanned using Illumina BeadArray Reader (part of Illumina BeadStation 500), according to the manufacturer's protocol.
P-MTAB-26183
labeling
150 ng of RNA was amplified and labelled using Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, USA) according to the manufacturer's protocol.
P-MTAB-26181
grow
RNA for the analysis was harvested from HF, HFcoHK, HFcoHaCaT and HFcoFaDu cultured for 7 days. Two parallel cultivations (9,4 cm2 culture dish) for each cell type were made. In the stage of confluency, the culture medium was removed and 360 ul of RTL lysis buffer (QIAGEN Inc., USA) was added. The cells were homogenized, transferred to a tube and quickly frozen in liquid nitrogen. The samples were stored at -80 degrees C.
P-MTAB-26182
nucleic_acid_extraction
Total RNA was isolated using RNeasy Micro Kit (Qiagen) and checked for integrity (RIN).
P-MTAB-26184
hybridization
1.5 microgram of cRNA per hybridisation was hybridised on Illumina HumanWG-6 v2 Expression BeadChip (Illumina, USA) according to the manufacturer's protocol (in Illumina Hybridization Oven).
P-MTAB-26180
specified_biomaterial_action
The cells were cocultured using a transwell system (Corning, Massachusetts, USA), in which the cell populations were separated by a microporous membrane preventing their direct intercellular contact. HF were cultured in 6 well plates and cancer epithelial cells (FaDu) were seeded into collagen-coated inserts in various densities respecting their specific growth kinetics (HF 1,000 cells/cm2, FaDu 2,000 cells/cm2). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Biochrom, Berlin, Germany) with 10% fetal bovine serum (FBS, Biochrom, Berlin, Germany) at 37 degrees C and 5% CO2 for 1 week. Culture medium was changed three times per week.
P-MTAB-26179
specified_biomaterial_action
The cells were cocultured using a transwell system (Corning, Massachusetts, USA), in which the cell populations were separated by a microporous membrane preventing their direct intercellular contact. HF cells were cultured in 6 well plates and immortalised epithelial cells (HaCaT) were seeded into collagen-coated inserts in various densities respecting their specific growth kinetics (HF 1,000 cells/cm2, HaCaT 10,000 cells/cm2). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Biochrom, Berlin, Germany) with 10% fetal bovine serum (FBS, Biochrom, Berlin, Germany) at 37 degrees C and 5% CO2 for 1 week. Culture medium was changed three times per week.
P-MTAB-26177
specified_biomaterial_action
HF cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Biochrom, Berlin, Germany) with 10% fetal bovine serum (FBS, Biochrom, Berlin, Germany) at 37 degrees C and 5% CO2 for 1 week. Culture medium was changed three times per week.
P-MTAB-26178
specified_biomaterial_action
The cells were cocultured using a transwell system (Corning, Massachusetts, USA), in which the cell populations were separated by a microporous membrane preventing their direct intercellular contact. HF cells were cultured in 6 well plates and normal human keratinocytes (HK) were seeded into collagen-coated inserts in densities respecting their specific growth kinetics (HF 1,000 cells/cm2, HK 40,000 cells/cm2). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Biochrom, Berlin, Germany) with 10% fetal bovine serum (FBS, Biochrom, Berlin, Germany) at 37 degrees C and 5% CO2 for 1 week. Culture medium was changed three times per week.