E-MTAB-1064 - Transcription profiling by array of human dermal fibroblasts cultured under different conditions to study their activation by normal and cancer epithelial cells.
Released on 2 November 2013, last updated on 3 May 2014
We model processes of wound healing and tumor growth, by studying the effects of normal and cancer epithelial cells on normal fibroblasts and their reciprocal effect on normal keratinocytes in vitro. We find strong parallels between the two processes and compare our observations with transcriptional analysis of 24 clinical samples of squamous cell carcinoma. This dataset contains the tissue culture samples, for the clinical samples, see E-MTAB-1065. Normal human fibroblasts (HF) were cocultured with either normal HK or transformed epithelial cells (HaCaT and FaDu) and their expression profile was compared to the expression profile of HF cultured alone.
transcription profiling by array, co-expression, growth condition, in vitro
Barbora Dvorankova, Cestmir Vlcek, Hana Kovarova, Hans-Joachim Gabius, Hynek Strnad <firstname.lastname@example.org>, Jan Betka, Jan Motlik, Jan Paces, Jan Plzak, Karel Smetana, Jr <email@example.com>, Karla Jarkovska, Lukas Lacina, Martin Chovanec, Michal Kolar <firstname.lastname@example.org>, Pavol Szabo, Zdenek Cada, Zdenek Fik
Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses. Kolář M, Szabo P, Dvořánková B, Lacina L, Gabius HJ, Strnad H, Sáchová J, Vlček C, Plzák J, Chovanec M, Cada Z, Betka J, Fík Z, Pačes J, Kovářová H, Motlík J, Jarkovská K, Smetana K Jr . Biology of the Cell 104(12):738-751 (2012), Europe PMC 23043537