E-MTAB-1019 - Transcription profiling by array of liver from C57BL6 mice transfected with AAV8 vectors expressing either GFP or human macrophage inhibitory factor (MIF) or none (PBS)
Released on 7 March 2013, last updated on 3 June 2014
MIF is a general mediator of inflammatory responses and is associated with several inflammatory diseases including atherosclerosis. Therefore MIF is currently investigated as a therapeutic target for atherosclerosis. Generation of AAV8 vectors expressing human MIF was initiated to overexpress human MIF in AAV8 target tissues (liver, heart, muscles) of C57/Bl6 to study biological function of MIF in vivo and to provide an animal model for testing of MIF antibodies. Aim of the gene expression profiling experiment: Livers of AAV8-CMV-MIF treated animals in order to identify established and potential novel target genes of MIF induced signalling
transcription profiling by array, co-expression, in vivo
Macrophage migration inhibitor factor is regulated in a peritonitis model but overexpression does not augment inflammation response. Andre Broermann, Annika Baur, Peter Maier, Norbert Redemann, Gerd Luippold, Tobias Hildebrandt, Eric Simon, Sebastian Kreuz, Jörg F. Rippmann.