E-MTAB-1001 - Microarray expression profiling of the Igf2 pathway dependency of Trp53 developmental and tumour phenotypes in mice
Submitted on 31 August 2010, released on 1 June 2012, last updated on 3 May 2014
We tested whether loss of p53 function leads to insulin-like growth factor 2 (IGF2) pathway dependency in vivo in genetically defined mouse models. Unexpectedly we found lethality, due to post-natal lung haemorrhage, occurred in Igf2 paternal null allele female mice (Igf2-p) but only if derived from double heterozygote fathers (Igf2-p, p53+/-). Consequently we investigated the effects of paternal genotype (wild-type, Igf2-p and Igf2-p, p53+/-) on gene expression. Microarray gene expression profiling of whole mouse embryos (embryonic day 9.5) revealed that lethality was associated with a specific gene signature. Since double heterozygote female offspring (Igf2-p, p53+/-) of Igf2-p, p53+/- fathers did not have the lethality phenotype we identified gene expression changes associated with this 'rescue'. Supplementary information available when browsing all available files.
transcription profiling by array, co-expression, development or differentiation, genetic modification, genotype, individual genetic characteristics, replicate
Andrew B Hassan <firstname.lastname@example.org>, Christopher F Graham <email@example.com>, Claudia Buehnemann <firstname.lastname@example.org>, David J Barnes <email@example.com>, Dilair Baban <firstname.lastname@example.org>, Emma J Carter <email@example.com>, Francesco Pezzella <firstname.lastname@example.org>, Ionel Sandovici <email@example.com>, Julien Bauer <firstname.lastname@example.org>, Miguel Constancia <email@example.com>, Victoria L Haley <V.Haley@bsms.ac.uk>
Igf2 pathway dependency of the Trp53 developmental and tumour phenotypes. Victoria L. Haley, David J. Barnes, Ionel Sandovici, Miguel Constancia, Christopher F. Graham, Francesco Pezzella, Claudia Buehnemann, Emma Carter, A. Bassim Hassan.