E-MEXP-935 - Transcription profiling of human ovarian cancer tumor samples to investigate heterogeneity
Submitted on 12 December 2006, released on 1 February 2007, last updated on 2 May 2014
Women that underwent operation for what was expected to be epithelial ovarian cancer at Odense University Hospital, Denmark, from April 2004 to February 2005, were consecutively asked to participate in the study, and all gave written consent. In the operating theatre the tumors were removed as an entirety, and immediately transported to the pathology department without fixation. One senior pathologist was responsible for collection of samples. From each tumor three samples of about 200 – 300 mg each were taken with maximal mutual distance, taking into consideration that all samples should contain tumor tissue. This way of sampling was done without any kind of magnification and is mentioned as macro-dissection. The three samples were given the letters a, b and c. The three samples from each of the nine tumors are compared.
transcription profiling by array, co-expression, disease state, replicate
Gene expression in epithelial ovarian cancer: a study of intratumor heterogeneity. K.M. Jochumsen, Q. Tan, B. Hølund, T.A. Kruse, O. Mogensen. Int J Gynecol Cancer (2007), Europe PMC 17367315