E-MEXP-842 - Transcription profiling of mouse liver cells with a conditionally active liver clock to descriminate between rhythmic gene expression driven by cyclic systemic cues and local oscillators
Released on 30 December 2006, last updated on 2 May 2014
The mammalian circadian timing system consists of a master pacemaker in the suprachiasmatic nucleus (SCN) that synchronizes self-sustained oscillators in most peripheral cells. Rhythmic gene expression in peripheral tissues can be driven by cyclic systemic cues emanating from the SCN or by local oscillators. To discriminate between these two mechanisms, we engineered a mouse strain with a conditionally active liver clock. Transcriptome profiling revealed that the circadian transcription of most genes depends on functional hepatocyte clocks. However, the expression of 31 genes, including mPer2, oscillates robustly in clock-arrested hepatocytes. Such genes may be implicated in the synchronization of liver oscillators
transcription profiling by array, compound treatment, innate behavior, time series
Olivier Schaad <Olivier.Schaad@biochem.unige.ch>, Benoît B Kornmann, Hermann H Bujard, Joseph S. J Takahashi, Ueli U Schibler
Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators. Bertolucci C, Cavallari N, Colognesi I, Aguzzi J, Chen Z, Caruso P, Foa A, Tosini G, Bernarid F, Pinotti M.
System-driven and oscillator-dependent circadian transcription in mice with a conditionally active liver clock. Kornmann B, Schaad O, Bujard H, Takahashi JS, Schibler U. :e34 (2007), Europe PMC 17298173