E-MEXP-817 - RNAi knock down of VCP in human HeLa cells

Status
Submitted on 3 August 2006, released on 23 August 2006, last updated on 2 May 2014
Organism
Homo sapiens
Samples (15)
Array (1)
Protocols (7)
Description
Study of the effects of the VCP knockdown. VCP (p97, yeast cdc48) is a hexameric AAA ATPase involved in various cellular functions including degradation of proteins by the ubiquitin-proteasome system. We examine the consequences of the reduction of VCP levels after RNAi of VCP in HeLa cells. We find ~30 transcripts upregulated in a sequence independent manner. Those transcripts encode proteins involved in endoplasmic reticulum stress, apoptosis, and amino acid starvation.
Experiment types
RNAi profiling by array, cellular modification, dye swap, reference, replicate
Contacts
Maga Rowicka <maga@work.swmed.edu>, Andrzej Kudlicki, Cezary Wojcik, Dominika Nowis, George Adams, George N DeMartino, Marek Kujawa
Citation
Valosin-containing Protein (p97) Is a Regulator of Endoplasmic Reticulum Stress and of the Degradation of N-End Rule and Ubiquitin-Fusion Degradation Pathway Substrates in Mammalian Cells. Wojcik, Cezary; Rowicka, Maga; Kudlicki, Andrzej; Nowis, Dominika; McConnell, Elizabeth; Kujawa, Marek; DeMartino, George N. Mol Biol Cell 17(11):4606 (2006), Europe PMC 16914519
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-817.idf.txt
Sample and data relationshipE-MEXP-817.sdrf.txt
Raw data (1)E-MEXP-817.raw.1.zip
Array designA-BASE-3.adf.txt
R ExpressionSetE-MEXP-817.eSet.r
Links