E-MEXP-765 - Transcription profiling by array of human mammary epithelial cells grown in 2D monolayers with or without EGF and insulin, or 3D Matrigel culture, after treatment with 4-hydroxytamoxifen
Released on 31 December 2006, last updated on 2 May 2014
MCF10A MycER cells were grown either on 2D monolayer culture in the presence of complete growth medium, in the absence of EGF and insulin or in 3D Matrigel culture for 20 days to form acinar structures. MycER was activated with 4-hydroxytamoxifen (4-OHT) in all samples for 2 or 48 h. Control cells were treated with ethanol. The aim of the study was to explore the effect of different growth conditions on gene expression changes in response to c-Myc activation.
transcription profiling by array, compound treatment, growth condition, time series