E-MEXP-754 - Transcription profiling of Arabidopsis seedlings under conditions of salt stress for 6, 24 and 48 hours
Released on 23 August 2006, last updated on 2 May 2014
Hydroponic cultured Arabidopis thaliana(ecotype Col-0) seeldings(18 days after germination)were treated with 1/2 X MS supplemented with 150 mM NaCl for 6, 24 and 48h, with roots harvested and pooled for RNA extraction. Control roots (1/2 X MS only)were also harvested at the same time for each time-point. 3 independent biological replicates were prepared for each time-point. Altogether 18 hybridizations including dye flips were performed with Qiagen Operon V1.0.2 26k microarray. Microarray data were analyzed using TIGR TM4 suite.
transcription profiling by array, compound treatment, dye swap, growth condition, time series
Yuanqing Jiang <email@example.com>, Michael Deyholos
Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes. Jiang, Yuanqing; Deyholos, Michael K.