E-MEXP-71 - Transcription profiling of light and carbon signaling interactions in Arabidopsis

Status
Released on 23 January 2004, last updated on 27 March 2012
Organism
Arabidopsis thaliana
Samples (8)
Array (1)
Protocols (10)
Description
A. thaliana seeds of Columbia (Col-0) ecotype were surface-sterilized, plated on designated media and vernalized for 48 hours at 8C. Plants were grown semi-hydroponically under 16-hr-light, 8-hr-dark cycles at a constant temperature of 23C on basal Murashige and Skoog medium supplemented with 2 mM KNO3, 2 mM NH4NO3 and 30 mM sucrose. Two-week-old seedlings were transferred to fresh MS media without nitrogen and sucrose and dark-adapted for 48 hours. To perform specific metabolic treatments (-C-L, +C-L, -C+L, +C+L), two-week-old seedlings were transferred to fresh MS medium containing 0% or 1% sucrose and either placed back into the dark or illuminated with white light for an additional 8 hours. The various treatments were compared to one another using the treatment of -C-L as the background treatment to which all other treatments (-C+L, +C-L, +C+L) were compared.
Experiment types
transcription profiling by array, growth condition
Contacts
Karen E Thum <karen.thum@gmail.com>, Andrei Kouranov, Gloria M Coruzzi, Michael J Shin, Peter M Palenchar
Citation
Genome-wide investigation of light and carbon signaling interactions in Arabidopsis. Karen Thum; Michael Shin; Peter Palenchar; Andrei Kouranov; Gloria Coruzzi.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-71.idf.txt
Sample and data relationshipE-MEXP-71.sdrf.txt
Raw data (1)E-MEXP-71.raw.1.zip
Array designA-AFFY-16.adf.txt
R ExpressionSetE-MEXP-71.eSet.r
Links