E-MEXP-708 - Transcription profiling of human HeLa cells synchronized at the G1-S boundary and then sampled at 2-hour intervals during S-phase
Submitted on 29 April 2006, released on 2 November 2006, last updated on 10 June 2011
The experimental strategy adopted to map this profile involved isolation of replication products from HeLa cells synchronized at the G1-S boundary by thymidine-aphidicolin double block. Cells released from the block were labeled with BrdU at every two-hour interval of the 10 hours of S-phase and DNA was isolated from them. The heavy-light(H/L) DNA representing the pool of DNA replicated during each two-hour labeling period was separated from the unlabeled DNA by double cesium chloride density gradient centrifugation. The purified heavy-light DNA was then hybridized to a high-density genome-tiling Affymetrix array comprised of all unique probes within the ENCODE regions.
transcription profiling by array, cell cycle, comparative genome hybridization, time series
Anindya Dutta <email@example.com>, Ankit Malhotra, Christopher Taylor, Neerja Karnani
PAN-S REPLICATION PATTERNS AND CHROMOSOMAL DOMAINS. Neerja Karnani; Christopher Taylor; Ankit Malhotra; Anindya Dutta. Genome Res (2006)