E-MEXP-708 - Transcription profiling of human HeLa cells synchronized at the G1-S boundary and then sampled at 2-hour intervals during S-phase

Status
Submitted on 29 April 2006, released on 2 November 2006, last updated on 10 June 2011
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (5)
Description
The experimental strategy adopted to map this profile involved isolation of replication products from HeLa cells synchronized at the G1-S boundary by thymidine-aphidicolin double block. Cells released from the block were labeled with BrdU at every two-hour interval of the 10 hours of S-phase and DNA was isolated from them. The heavy-light(H/L) DNA representing the pool of DNA replicated during each two-hour labeling period was separated from the unlabeled DNA by double cesium chloride density gradient centrifugation. The purified heavy-light DNA was then hybridized to a high-density genome-tiling Affymetrix array comprised of all unique probes within the ENCODE regions.
Experiment types
transcription profiling by array, cell cycle, comparative genome hybridization, time series
Contacts
Anindya Dutta <ad8q@virginia.edu>, Ankit Malhotra, Christopher Taylor, Neerja Karnani
Citation
PAN-S REPLICATION PATTERNS AND CHROMOSOMAL DOMAINS. Neerja Karnani; Christopher Taylor; Ankit Malhotra; Anindya Dutta. Genome Res  (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-708.idf.txt
Sample and data relationshipE-MEXP-708.sdrf.txt
Raw data (1)E-MEXP-708.raw.1.zip
Array designA-AFFY-61.adf.txt
R ExpressionSetE-MEXP-708.eSet.r
Links