E-MEXP-637 - Transcription profiling by array of Arabidopsis mutant for brx after treatment with brassinolide or indole-3-acetic acid

Status
Released on 28 September 2006, last updated on 2 May 2014
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (6)
Description
We compared the seedling transcription profiles to determine the effects of loss-of-function of the BRX gene of Arabidopsis. BRX is required for optimal root growth. We compared seedlings of a loss-of-function line (brx) with its control background (Sav-0). Because the loss-of-function line was derived from introgression, a brx line that was complemented by a transgenic wild type copy of BRX was also included as a control. This line (rescued brx) allows the identification of expression differences that are due to introgression drag. See Mouchel et al. 2004, Genes & Dev. Vol. 18, p. 700 for a detailed description. We also compared to response of the different genotypes to the application of the phytohormones brassinolide (BL) and indole acetic acid (IAA)
Experiment types
transcription profiling by array, compound treatment, strain or line
Contacts
Christian Hardtke <christian.hardtke@unil.ch>, Céline F Mouchel, Karen S Osmont
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-637.idf.txt
Sample and data relationshipE-MEXP-637.sdrf.txt
Raw data (1)E-MEXP-637.raw.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-MEXP-637.eSet.r
Links