E-MEXP-580 - Transcription profiling by array of Saccharomyces cerevisiae mutant for YHB1 after treatment with DETA NONOate

Status
Released on 2 March 2006, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (15)
Array (1)
Protocols (7)
Description
Nitric oxide and NO-derived species (RNS) are defense molecules with broad antimicrobial activity. Micro-organisms have developed strategies to sense RNS and counteract their damaging effects. We used Saccharomyces cerevisiae, harbouring a deletion of YHB1 that encodes the main NO scavenger enzyme, to study consequences of RNS exposure on whole genome transcriptional response. The expression of >700 genes was altered on RNS treatment. No major role for ROS-scavenging enzymes was found, and the respiratory chain, the main site of ROS production, had only minor involvement in the RNS-induced stress. The changes were generally transient and also found after treatment with the respiratory inhibitor myxothiazol. 117 genes however showed a persistent response which was not observed after myxothiazol treatment. Of these, genes of the glutathione and DNA repair systems, iron homeostasis and transport were found up-regulated. Severe repression of genes of respiratory chain enzymes was observed. Many of these genes are known to be regulated by the transcription factor Hap1p suggesting that RNS might interfere with Hap1p activity. We showed also that Msn2/4p and Yap1p, key regulators of the response to, respectively, general stress and oxidative stress, played a role in mediating the RNS-induced response.
Experiment types
transcription profiling by array, compound treatment, genetic modification
Contacts
INGRID BOURGES <ibourges@hotmail.com>, BRIGITTE MEUNIER, SUSANNAH HORAN
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-580.idf.txt
Sample and data relationshipE-MEXP-580.sdrf.txt
Raw data (1)E-MEXP-580.raw.1.zip
Processed data (1)E-MEXP-580.processed.1.zip
Array designA-AFFY-27.adf.txt
R ExpressionSetE-MEXP-580.eSet.r
Links