E-MEXP-566 - Transcription profiling of primary human tubular epithelial cells (HUTEC) response to TGFbeta at three concentrations to study the epithelial to mesenchymal transition

Status
Submitted on 9 February 2006, released on 31 December 2006, last updated on 2 May 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (10)
Description
This experiment was aimed to study the Epithelial to mesenchimal transition (EMT) induced by TGFbeta in primary tubular epithelial cells. Primary cultures were grown for 4 days using three different TGFbeta1 concentrations: 5 ng/ml, 10 ng/ml and 50 ng/ml. Cells were grown in plastic flasks (Falcon) or in plastic flasks (Falcon) covered by type I collagen. Differentially expressed genes were identified comparing treated vs. control cells maintained for 4 days in 1% serum without TGFbeta1.
Experiment types
transcription profiling by array, dose response
Contact
Citation
Genes involved in TGF beta1-driven epithelial-mesenchymal transition of renal epithelial cells are topologically related in the human interactome map. Campanaro S, Picelli S, Torregrossa R, Colluto L, Ceol M, Del Prete D, DAngelo A, Valle G, Anglani F. BMC Genomics 8:383 (2007), Europe PMC 17953753
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-566.idf.txt
Sample and data relationshipE-MEXP-566.sdrf.txt
Raw data (1)E-MEXP-566.raw.1.zip
Array designA-MAXD-4.adf.txt
R ExpressionSetE-MEXP-566.eSet.r
Links