E-MEXP-548 - RNAi knock down of RNA binding IMP proteins in human epithelial (HeLa) cells

Status
Released on 30 September 2006, last updated on 3 June 2014
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (8)
Description
To identify factors and pathways regulated by IMP proteins and obtain leads to the mechanism behind the phenotypic changes, we compared the gene expression profiles of IMP siRNA treated cells with mock treated cells. Triplicate gene expression profiles were generated from both the IMP(1,3)A and IMP(1,3)B siRNA sets and were compared to the mock transfected cells. cRNA was hybridized to Affymetrix human U133A arrays.
Experiment types
transcription profiling by array, RNAi profiling by array, cellular modification
Contacts
Rehannah H Borup <rehannah.borup@rh.hosp.dk>, Jonas Vikesaa, Thomas O Hansen
Citation
RNA-binding IMPs promote cell adhesion and invadopodia formation. Jonas Vikesaa, Thomas Hansen, Lars Jonson, Rehannah Borup, Ulla M Wewer, Jan Christiansen and Finn C Nielsen.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-548.idf.txt
Sample and data relationshipE-MEXP-548.sdrf.txt
Raw data (1)E-MEXP-548.raw.1.zip
Processed data (1)E-MEXP-548.processed.1.zip
Array designA-AFFY-33.adf.txt
Links