E-MEXP-491 - Transcription profiling of ethylene-dependent responses to short day exposure in birch apices

Status
Submitted on 15 November 2005, released on 23 November 2005, last updated on 3 May 2014
Organism
Betula pendula
Samples (9)
Array (1)
Protocols (10)
Description
We have used transgenic ethylene-insensitive birches (Betula pendula), which express the Arabidopsis ethylene receptor gene ETR1 carrying the dominant mutation etr1-1, to investigate the role of ethylene in short day (SD) -induced responses in the shoot apical meristem in birch. Wild-type birch (clone V5834) and two ethylene-insensitive lines in this background (BPetr1-1-35 and BPetr1-1-86; see Plant Physiol 132: 185-195) were exposed to SD. After 12, 16 and 20 days under SD, apices of branches of three trees were pooled before RNA extraction from each sample. To study the ethylene-dependent SD-transcriptome in birch apices, the RNA extracts of lines BPetr1-1-35 and BPetr1-1-86 were separately compared with the reference, wild-type V5834, at the three time points (12SD, 16SD, 20SD) resulting in altogether six microarray hybridizations.
Experiment types
transcription profiling by array, growth condition
Contact
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-491.idf.txt
Sample and data relationshipE-MEXP-491.sdrf.txt
Raw data (1)E-MEXP-491.raw.1.zip
Array designA-MEXP-93.adf.txt
Links