E-MEXP-471 - Chromatin immunoprecipitation of human cell lines with oestrogen receptor (ER), the E2F transcription factor 4 (E2F4),
Released on 1 May 2006, last updated on 27 March 2012
The Goal of the experiment was to validate the predicted regulatory modules identified by an algorithm.For this we took advantage of the the genome-wide location analysis technique (or ?ChIP/chip?). We selected modules predicted to be bound by the estrogen receptor (ER), the E2F transcription factor 4 (E2F4), the signal transducer and activator of transcription 3 (STAT3), and the hypoxia-inducible factor 1 HIF1), to print a DNA microarray. In the current study, the microarray was then probed by ChIP/chip for ER and E2F4.
ChIP-chip by array, binding site identification, unknown experiment type
Fran�ois Robert <Francois.Robert@ircm.qc.ca>, Alain R Bataille, Benoit Coulombe, Christian Poitras, CŽline Lef�bvre, Dominique Bergeron, Genevi�ve Deblois, JosŽe Lagani�re, Mathieu Blanchette, Vincent Ferretti, Vincent Gigu�re, Xiaoyu Chen
Genome-wide computational prediction of transcriptional regulatory modules reveals new insights into human gene expression. Mathieu Blanchette; Alain Bataille; Xiaoyu Chen; Christian Poitras; JosŽe Lagani�re; CŽline Lef�bvre; Genevi�ve Deblois; Vincent Gigu�re; Vincent Ferretti; Dominique Bergeron; Benoit Coulombe; Fran�ois Robert.