E-MEXP-461 - Transcription profiling of human Ki-ras transformed embryo prostate epithelial cells (267B1) to identify mRNAs under differential translational control

Status
Released on 26 June 2007, last updated on 3 May 2014
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (7)
Description
The purpose of the experiment is to identify mRNAs under differential translational control in Ki-ras transformed 267B1 cells as compared to the parental cell line. Total and polysome-associated mRNAs were isolated from exponentially growing cells and used to prepare biotin-labeled cRNA probes for analysis on Affymetrix microarray chips as described previously. The Data Mining tool (Affymetrix) was used to identify mRNAs that were differentially increased or decreased only in polysome fractions.
Experiment types
transcription profiling by array, translational bias
Contact
Citation
Messenger RNAs under differential translational control in Ki-ras-transformed cells. Jean Spence, Brendan M. Duggan, Colleen Eckhardt, Michael McClelland, and Dan Mercola. Mol Cancer Res 4(1):47-60 (2006), Europe PMC 16446406
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-461.idf.txt
Sample and data relationshipE-MEXP-461.sdrf.txt
Raw data (1)E-MEXP-461.raw.1.zip
Processed data (1)E-MEXP-461.processed.1.zip
Array designA-AFFY-1.adf.txt
R ExpressionSetE-MEXP-461.eSet.r
Links