E-MEXP-461 - Transcription profiling of human Ki-ras transformed embryo prostate epithelial cells (267B1) to identify mRNAs under differential translational control
Released on 26 June 2007, last updated on 3 May 2014
The purpose of the experiment is to identify mRNAs under differential translational control in Ki-ras transformed 267B1 cells as compared to the parental cell line. Total and polysome-associated mRNAs were isolated from exponentially growing cells and used to prepare biotin-labeled cRNA probes for analysis on Affymetrix microarray chips as described previously. The Data Mining tool (Affymetrix) was used to identify mRNAs that were differentially increased or decreased only in polysome fractions.
transcription profiling by array, translational bias
Messenger RNAs under differential translational control in Ki-ras-transformed cells. Jean Spence, Brendan M. Duggan, Colleen Eckhardt, Michael McClelland, and Dan Mercola. Mol Cancer Res 4(1):47-60 (2006), Europe PMC 16446406