E-MEXP-459 - Transcription profiling of mouse primary microglial cultures with and without neurovirulent (FrCasE) and non-neurovirulent (Fr57E) virus infection

Status
Released on 11 May 2005, last updated on 3 May 2014
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (10)
Description
We undertook a survey of gene expression changes in primary microglial cultures with and without neurovirulent (FrCasE) and non-neurovirulent (Fr57E) virus infection to identify physiological changes that could be relevant to the induction of spongiform neurodegeneration. These gene expression analyses were performed using Affymetrix 430A mouse GeneChips (5 chips for each of the three experimental conditions, representing over 14,000 murine genes and ESTs. RNA from 5 separate microglial culture preparations were analyzed for Control (mock infected), Fr57E-, and FrCasE-infected microglia. Present/absent calls were based on MicroArray Suite 5.0 from Affymetrix. Affymetrix CEL files were analyzed using dChip software after normalization of the data between all 15 arrays. Statistical analyses were performed using ANOVA.
Experiment types
transcription profiling by array, disease state
Contacts
William P Lynch <wonk@neoucom.edu>, Angelo L DeLucia, Derek E Dimcheff, L. Gwenn Volkert, Ying Li
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-459.idf.txt
Sample and data relationshipE-MEXP-459.sdrf.txt
Raw data (1)E-MEXP-459.raw.1.zip
Processed data (1)E-MEXP-459.processed.1.zip
Array designA-AFFY-23.adf.txt
R ExpressionSetE-MEXP-459.eSet.r
Links