E-MEXP-450 - Transcription profiling of imprint free mouse mouse embryonic stem (IF-ES) cells vs wild type MEFs
Submitted on 6 October 2005, released on 20 October 2005, last updated on 3 May 2014
Loss of imprinting (LOI), commonly observed in human tumors, refers to loss of monoallelic gene regulation normally conferred by parent-of-origin specific DNA methylation. To test the function of LOI in tumorigenesis, we developed a model using transient demethylation to generate imprint-free mouse embryonic stem (IF-ES) cells. To analyse the effect of LOI on gene expression, we compared gene expression profiles from embryonic fibroblasts derived from IF-ES cells (IF-MEFs) and wildtype MEFs.
transcription profiling by array, genetic modification
Tobias Brambrink <email@example.com>, Laurie Jackson-Grusby, Rudolf Jaenisch, Teresa M Holm, William M Rideout, Yasuhiro Yamada
Global Loss of Imprinting Leads to Widespread Tumorigenesis in Adult Mice. Teresa Holm; Laurie Jackson-Grusby; Tobias Brambrink; Yasuhiro Yamada; William Rideout; Rudolf Jaenisch. Cancer Cell 8(4):275ñ285 (2005)