E-MEXP-450 - Transcription profiling of imprint free mouse mouse embryonic stem (IF-ES) cells vs wild type MEFs

Status
Submitted on 6 October 2005, released on 20 October 2005, last updated on 3 May 2014
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (5)
Description
Loss of imprinting (LOI), commonly observed in human tumors, refers to loss of monoallelic gene regulation normally conferred by parent-of-origin specific DNA methylation. To test the function of LOI in tumorigenesis, we developed a model using transient demethylation to generate imprint-free mouse embryonic stem (IF-ES) cells. To analyse the effect of LOI on gene expression, we compared gene expression profiles from embryonic fibroblasts derived from IF-ES cells (IF-MEFs) and wildtype MEFs.
Experiment types
transcription profiling by array, genetic modification
Contacts
Tobias Brambrink <brambrink@wi.mit.edu>, Laurie Jackson-Grusby, Rudolf Jaenisch, Teresa M Holm, William M Rideout, Yasuhiro Yamada
Citation
Global Loss of Imprinting Leads to Widespread Tumorigenesis in Adult Mice. Teresa Holm; Laurie Jackson-Grusby; Tobias Brambrink; Yasuhiro Yamada; William Rideout; Rudolf Jaenisch. Cancer Cell 8(4):275ñ285 (2005)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-450.idf.txt
Sample and data relationshipE-MEXP-450.sdrf.txt
Raw data (1)E-MEXP-450.raw.1.zip
Array designA-MEXP-264.adf.txt
R ExpressionSetE-MEXP-450.eSet.r
Links