E-MEXP-448 - Platform and RNA amplification method comparisons using Universal Human Reference RNA and Caki-1 RNA on Agilent Human 1A Oligo microarrays and Affymetrix HG-U133Plus 2.0 GeneChips
Submitted on 22 September 2005, released on 17 May 2006, last updated on 3 May 2014
The profiles of gene transcripts from 3 indipendent samples of Universal Human Reference RNA and Caki-1 RNA, amplified into biotinylated cRNA according to 1) standard Affymetrix procedure 'one-cycle target labeling' 2) standard Affymetrix procedure 'two cycle target labeling' 3) Transplex WTA method, were hybridized on HG-U133Plus 2.0 GeneChips and compared. In addition we performed the same gene expression comparison using not amplified HHR and Caki1 RNA samples on Agilent Human 1A Oligo microarrays.
transcription profiling by array, optimization