8 protocols
AccessionType
array scanning protocol
The scanning was performed according to the manufacturer's protocol (Agilent technologies, A Method for Quantifying the performance of a DNA Microarray Scanner, Publication Number 5988-9498EN). (Parameters: Scanning hardware = DNA Microarray Scanner BA [Agilent Technologies], Scanning software = Feature Extraction Software [Agilent])
nucleic acid hybridization to array protocol
The hybridization experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Chamber type = OTHER: Robbins Scientific 1040-60-1AG, Quantity of label target used = 1, Mass unit = Micro gram, Tiny time unit = seconds, Volume unit = Nano litre, temperature = 65)
nucleic acid labeling protocol
The labeling experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
nucleic acid labeling protocol
The labeling experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
growth protocol
Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07–1.08) under a 16 h light/8 h dark regimen (40 ± 10 umol photons/m2/s) at 22C. (Parameters: start time = 3, time unit = weeks, min temperature = 22, temperature unit = C, media = soil)
nucleic acid extraction protocol
Total RNAs were isolated using the RNAiso Reagent (Takara, Japan). (Parameters: Extracted product = total_RNA, Amplification = none)
treatment protocol
The plants were incubated at 22C under non-stress condition.
treatment protocol
The 3-week-old plants were transferred from 22C to 4C and were grown for 1 or 4 days.