6 protocols
AccessionNameType
P-AGIL-2
hybridization
Agilent One-Color Microarray-Based Gene Expression (Hybridization) Version: 5.7 Agilent publication number: G4140-90040 URL: http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_One-Color_GE_5.7.pdf This document describes Agilent's recommended procedures for the preparation and labeling of complex biological targets and hybridization, washing, scanning, and feature extraction of Agilent 60-mer oligonucleotide microarrays for microarray-based one-color gene expression analysis.
P-AGIL-28
labeling
P-AGIL-28
P-MTAB-35296
array scanning protocol
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (Agilent G2565AA and Agilent G2565BA) using one color scan setting for 4x44k array slides ( Scan resolution 5um, Dye channel is set to Green and Green PMT is set to 100%). (Parameters: Scanning hardware = G2565BA DNA microarray scanner [Agilent], Scanning software = Feature Extraction Software [Agilent])
P-MTAB-35298
nucleic acid extraction protocol
Total RNA was isolated from frozen LD samples using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions and dissolved in RNase-free water. Total RNA concentration was assessed by spectrophotometry (OD 260 nm) and adjusted to a final concentration of 2.0ug/ul. The integrity and purity of the RNA were determined by the absorbance ratio at 260/280 nm and 2100 RIN. (Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-35295
treatment protocol
After birth,the longissimus dorsi (LD) samples were collected from the newborn piglets immediately, and washed briefly with PBS before being frozen in liquid nitrogen for detection.
P-MTAB-35297
growth protocol
In the FD group, standard pig feed without additional folate was provided to the pregnant sows from pregnancy.In the N group, the sows were fed standard pregnant sow feed (NRC 1998) with 1.3 mg/kg of added folate; this meets the folate requirement of pregnant sows. After 60 days of gestation, the sows were fed an ordinary feed designed for late pregnant sows. (Parameters: time unit = seconds, temperature unit = C)