E-MEXP-3990 - Effects of SocB toxin on genome-wide expression in Caulobacter crescentus
Released on 10 December 2013, last updated on 3 June 2014
Caulobacter cells were grown to exponential phase in rich PYE medium. At time zero, expression of socB-M2 was induced by the addition of 0.3% xylose. A reference sample was also collected at this time point. Atter 1.5 hours, the experimental sample was then collected. RNA was extracted from the reference and experimental samples, reverse transcribed into Cy3- or Cy5-labeled cDNA, and then hybridized to Agilent 4x44K Caulobacter arrays.
transcription profiling by array, co-expression, in vivo, stimulus or stress design
A Bacterial Toxin Inhibits DNA Replication Elongation Through a Direct Interaction with the beta Sliding Clamp.