E-MEXP-3972 - Transcription profiling of young and old yeast cells grown in pH controlled and uncontrolled media
Released on 12 January 2014, last updated on 3 June 2014
A 2 x 2 factorial design was used to elucidate the genome-wide transcriptional responses of old and young cells to the medium pH control. ?HO strain (BY4742 background) was cultivated batch-wise in SDC media and in fully controlled fermenters. pH was maintained at 4.5 via automatic NaOH addition for pH controlled case while this control was turned off for the unbuffered experiments. Samples of the young and old cells, collected at the 3rd and 7th day of the experiment respectively, were transferred to stimulating media containing fresh SDC medium prior to sample collection for transcriptional profiling.
transcription profiling by array, co-expression, growth condition design, reference design, replicate design, stimulus or stress design
The Impacts of Medium Acidity on Chronological Life Span of S. cerevisiae: Lipids, Signaling Cascades, Mitochondrial and Vacuolar Functions.