E-MEXP-3970 - Transcriptional response of Pseudomonas aeruginosa PAO1 to human host defense peptide LL-37

Status
Released on 20 December 2013, last updated on 3 June 2014
Organism
Pseudomonas aeruginosa
Samples (4)
Array (1)
Protocols (6)
Description
In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 µg/ml) or without peptide for 2 h at 37 °C following RNA extraction and microarray analysis.
Experiment types
transcription profiling by array, co-expression, compound treatment design, in vitro
Contact
Citation
Human Host Defense Peptide LL-37 Stimulates Virulence Factor Production and Adaptive Resistance in Pseudomonas aeruginosa. Strempel N, Neidig A, Nusser M, Geffers R, Vieillard J, Lesouhaitier O, Brenner-Weiss G, Overhage J. PLoS One 8(12):e82240 (2013), Europe PMC 24349231
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3970.idf.txt
Sample and data relationshipE-MEXP-3970.sdrf.txt
Raw data (1)E-MEXP-3970.raw.1.zip
Processed data (1)E-MEXP-3970.processed.1.zip
Array designA-AFFY-30.adf.txt
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