Title: Affymetrix CEL analysis. Description:
Affymetrix Generic Hybridization
normalization data transformation protocol
Data were normalized using the GCRMA method.
nucleic acid labeling protocol
Samples were hybridised to Mouse Genome 430 2.0 gene expression arrays (Affymetrix) at the Stanford Protein and Nucleic Acid facility. (Parameters: Amplification = none, Mass unit = Micro gram)
nucleic acid extraction protocol
BM was isolated from a pool of transgenic mice (8-12 weeks old) and 2000-3000 Vwf+ or Vwf- HSC were sorted directly into Trizol (Invitrogen) in two experiments. RNA extraction was carried out as per the manufacturer's instructions and amplification and labeling was performed using the WT-Ovation Pico RNA Amplification System (Nugen). (Parameters: Extracted product = total_RNA, Amplification = OTHER: WT-Ovation Pico RNA Amplification System (Nugen))