5 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Affymetrix:Protocol:Hybridization-Unknown
hybridization
P-MTAB-33762
normalization data transformation protocol
Data were normalized using the GCRMA method.
P-MTAB-33761
nucleic acid labeling protocol
Samples were hybridised to Mouse Genome 430 2.0 gene expression arrays (Affymetrix) at the Stanford Protein and Nucleic Acid facility. (Parameters: Amplification = none, Mass unit = Micro gram)
P-MTAB-33763
nucleic acid extraction protocol
BM was isolated from a pool of transgenic mice (8-12 weeks old) and 2000-3000 Vwf+ or Vwf- HSC were sorted directly into Trizol (Invitrogen) in two experiments. RNA extraction was carried out as per the manufacturer's instructions and amplification and labeling was performed using the WT-Ovation Pico RNA Amplification System (Nugen). (Parameters: Extracted product = total_RNA, Amplification = OTHER: WT-Ovation Pico RNA Amplification System (Nugen))