7 protocols
AccessionType
 
nucleic acid extraction protocol
RNA extracted by homogenization in Qiazol, followed by chloroform extraction and clean up with RNEasy kit (Qiagen)
(Parameters: Extracted product = total_RNA, Amplification = none)
array scanning protocol
GeneChip arrays were scanned using Affymetrix GeneChip Scanner 3000 7G.
(Parameters: Scanning hardware = OTHER: Affymetrix GeneChip Scanner 3000 7G, Scanning software = MAS/GCOS/GREX [Affymetrix])
nucleic acid hybridization to array protocol
5.5ug labelled cDNA were hybridized for 16hr at 45C on whole transcript GeneChip HuGene 1.0 ST Arrays using Hybridization Oven 640. The array were then washed using GeneChip Fludics station 450.
(Parameters: Chamber type = Affymetrix- GeneChip Hyb Oven 640, Quantity of label target used = 5.5, Mass unit = Micro gram, time = 16, Tiny time unit = hours, Volume unit = Nano litre, temperature = 45)
array scanning protocol
GeneChip arrays were scanned using Affymetrix GeneChip Scanner 3000 7G.
(Parameters: Scanning hardware = GeneChip Scanner 3000 [Affymetrix], Scanning software = AIDA [Raytest GmbH])
nucleic acid labeling protocol
cDNA is labelled by terminal deoxynucleotidy transferase (TdT) with Affymetrix proprietary DNA labelling kit from 10ugcRNA. The cRNA were amplified from 250ng total RNA (Affymetrix GeneChip Whole Transcript Expression arrays).
(Parameters: Amount of nucleic acid labeled = 10, Amplification = none, Mass unit = Micro gram)
treatment protocol