E-MEXP-392 - Transcription profiling of mouse ES cells: WT, HIF-1KO, HIF-2KO cells treated under 21% or 1.5% oxygen to indentify hypoxia induced genes

Status
Released on 1 July 2006, last updated on 3 May 2014
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (8)
Description
WT, HIF-1KO, HIF-2KO ES cells were treated under 21% or 1.5% oxygen. HIF-1 target genes will be identified in HIF-2KO ES cell while HIF-2 target genes will be identified in HIF-1KO ES cells. WT ES cells expressing both HIF-1 and HIF-2 protein will be useful to identify hypoxia indicible genes induced by HIF-1 and HIF-2.
Experiment types
transcription profiling by array, growth condition
Contacts
Cheng-Jun Hu <chenghu@mail.med.upenn.edu>, Aneesa Sataur, Celeste Simon, Covello Kelly, Lewis Chodosh, Sangeeta Iyer
Citation
Hypoxia-inducible factor 2 alpha is inhibited by a tissue-specific repressor in stem cells. Cheng-Jun Hu; Sangeeta Iyer; Aneesa Sataur; Covello Kelly; Lewis Chodosh; Celeste Simon.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-392.idf.txt
Sample and data relationshipE-MEXP-392.sdrf.txt
Raw data (1)E-MEXP-392.raw.1.zip
Processed data (1)E-MEXP-392.processed.1.zip
Array designA-AFFY-6.adf.txt
R ExpressionSetE-MEXP-392.eSet.r
Links