E-MEXP-3919 - Transcription profiling by array of E. coli cells before and after selection with histones from calf thymus to identify genes protecting from histone killing
Released on 4 June 2013, last updated on 3 June 2014
The experiment's aim is to follow the population of E. coli cells before and after treatment with histones. The E. coli libraries consisted of MACH1 pSMART-LCKm carrying random fragments of 1, 2, 4 and 8 kb of E. coli genome. The selection procedure involved three rounds of histones treatment (same dose each time during 3h hours then recovery time of 2h before applying the next histone treatment, at the end a total of 15-16h).
comparative genomic hybridization by array, comparative genome hybridization, compound treatment design, in vitro
O-antigen protects Gram-negative bacteria from histone killing.