E-MEXP-3919 - Transcription profiling by array of E. coli cells before and after selection with histones from calf thymus to identify genes protecting from histone killing

Status
Released on 4 June 2013, last updated on 3 June 2014
Organism
Escherichia coli
Samples (2)
Array (1)
Protocols (6)
Description
The experiment's aim is to follow the population of E. coli cells before and after treatment with histones. The E. coli libraries consisted of MACH1 pSMART-LCKm carrying random fragments of 1, 2, 4 and 8 kb of E. coli genome. The selection procedure involved three rounds of histones treatment (same dose each time during 3h hours then recovery time of 2h before applying the next histone treatment, at the end a total of 15-16h).
Experiment types
comparative genomic hybridization by array, comparative genome hybridization, compound treatment design, in vitro
Contact
Citation
O-antigen protects Gram-negative bacteria from histone killing.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3919.idf.txt
Sample and data relationshipE-MEXP-3919.sdrf.txt
Raw data (1)E-MEXP-3919.raw.1.zip
Array designA-AFFY-29.adf.txt
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