E-MEXP-3918 - Transcription profiling by array of Brachypodium response to increased ambient temperature in seedlings

Status
Released on 7 June 2013, last updated on 3 May 2014
Organism
Brachypodium distachyon
Samples (15)
Array (1)
Protocols (8)
Description
Microarray analysis on Brachypodium distachyon seedlings was performed to determine the response of the transcriptome to changes in ambient temperature, including identification of marker genes that were up-regulated or down-regulated by a moderate increase in growth temperature. Wild-type Brachypodium (Bd21) seedlings were grown on MSR63 media without sucrose (Alves et al. 2009 Nature Protocols vol. 4 pp 638-649) in a short-day photoperiod (14 hr light/ 10 hr dark) at 17 ºC. As the third leaf was emerging, plants were transferred to 12 ºC for 48 hrs. Plants were then maintained at 12 ºC or transferred to one of two temperature treatments: constant 22 ºC or constant 27 ºC. Samples were collected before the shift (0 hr) and at 2 hr and 24 hr after the shift and immediately frozen in liquid nitrogen. For each harvest, two to three replicates were collected that each contained 3 seedlings.
Experiment types
transcription profiling by array, co-expression, in vivo, stimulus or stress design
Contact
Citation
Thermal stress effects on grain yield in Brachypodium occur via H2A.Z-nucleosomes.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3918.idf.txt
Sample and data relationshipE-MEXP-3918.sdrf.txt
Raw data (2)E-MEXP-3918.raw.1.zip, E-MEXP-3918.raw.2.zip
Processed data (1)E-MEXP-3918.processed.1.zip
Array designA-AFFY-190.adf.txt
Links