E-MEXP-3903 - microRNA profiling by array of human liver sinusoidal endothelial cells (LSECs) treated with IFN-a

Status
Released on 17 May 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
LSECs were grown in culture medium supplemented with 10% FBS (depleted of endogenous exosomes by overnight centrifugation at 100,000 g) and were treated with or without 1,000 U/ml IFN-a (PBL Interferon Source, Piscataway, NJ, USA) for 48 h. The exosomes from the cell culture supernatants were isolated by differential centrifugation as follow, at 300g for 10 min, 2,000g for 10 min, 10,000g for 30 min, and 100,000g for 70 min, washed once with PBS, and purified by centrifugation at 100,000g for 70 min. Total RNA of purified exosomes were extracted and Exiqon miRCURY LNA Expression Array were used for detection of mRNA and miRNA, respectively.
Experiment types
microRNA profiling by array, co-expression, compound treatment design, in vitro, stimulated type
Contact
Citation
Exosomes mediate the cell-to-cell transmission of interferon alpha-induced antiviral activity.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3903.idf.txt
Sample and data relationshipE-MEXP-3903.sdrf.txt
Raw data (1)E-MEXP-3903.raw.1.zip
Processed data (2)E-MEXP-3903.processed.1.zip, E-MEXP-3903.processed.2.zip
Array designA-MEXP-2004.adf.txt
Links