E-MEXP-3891 - Transcription profiling by array of midguts from Apis mellifera to investigate the impact of Nosema apis infection on worker honey bee midgut gene expression
Released on 3 May 2014, last updated on 13 May 2014
Newly emerged adult workers (24 hours old) were infected with 50,000 Nosema apis spores in sucrose solution. Controls were fed sucrose. Workers were maintained in cages in an incubator and collected at 1 and 2 days post-infection. Midguts were dissected and whole genome expression in this tissue was compared across treatments and collection time points using microarrays.
transcription profiling by array, co-expression, disease state design, dye swap design, loop design, time series design
Chronic parasitization by the microsporidian Nosema causes global expression changes in core nutritional, metabolic and behavioral pathways in honey bee workers (Apis mellifera).