E-MEXP-3889 - Transcription profiling by array of eviscerated abdomens from Apis mellifera to investigate the impact of Nosema apis and Nosema ceranae co-infection on worker fat body gene expression
Released on 1 May 2014, last updated on 3 June 2014
Newly emerged adult workers (24 hours old) were infected with 25,000 Nosema apis spores and 25,000 Nosema ceranae spores in sucrose solution. Controls were fed sucrose. Workers were maintained in cages in an incubator and collected at 14 days post-infection. Fat bodies (eviscerated abdomens) were dissected and whole genome expression in this tissue was compared across treatments using microarrays.
transcription profiling by array, co-expression, disease state design, dye swap design, loop design
Chronic parasitization by the microsporidian Nosema causes global expression changes in core nutritional, metabolic and behavioral pathways in honey bee workers (Apis mellifera).