E-MEXP-3883 - Transcription profiling by array of mouse neural progenitor cells isolated from forebrains of E13.5 dpc mouse embryos with wild-type Miz1 or homozygous for Miz1-delta-POZ
Released on 1 October 2013, last updated on 3 June 2014
To isolate neuronal progenitor cells (NPCs), forebrains of E13.5 Miz1+/+ or Miz1-delta-POZ embryos were cut in small pieces, digested with trypsin and filtered through sterile gauze. Cells were cultivated in 2:1 DMEM/F12 supplemented with 1xB27 (Life technologies), 20 ng/ul EGF (Biomol), 20 ng/?l basic FGF (Biomol), 1 ug/ml fungizone (Gibco) and Penicillin/Streptomycin (PAA). NPCs were passaged every seven days. RNA expression of different genotypes was compared in sec. and quart. neurospheres.
transcription profiling by array, co-expression, genetic modification design, in vitro, time series design
Miz1 is required to maintain autophagic flux. Elmar Wolf,Anneli Gebhardt,Daisuke Kawauchi,Susanne Walz,Björn von Eyss,Nicole Wagner,Christoph Renninger,Georg Krohne,Esther Asan,Martine F Roussel,Martin Eilers. Nat Commun (2013)