E-MEXP-3872 - Transcription profiling by array of human BIN-67 small cell ovarian carcinoma to investigate gene expression

Status
Last updated on 23 September 2013, released on 24 September 2013
Organism
Homo sapiens
Samples (1)
Array (1)
Protocols (6)
Description
The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. Microarray expression analysis was performed using the GeneChip? Human Genome U133 Plus 2.0 Array (Affymetrix?, Santa Clara, CA) with total RNA from BIN-67 cells. Hybridization and scanning were performed at the McGill University and Genome Quebec Innovation Centre (Montreal, QC). Gene expression levels were determined from the scanned images using Affymetrix? Microarray Suite (MAS) version 5.0 software expression algorithm (Affymetrix?, Santa Clara, CA). Gene expression profiles were compared with Affymetrix U133 Plus 2.0 (MAS5.0) generated expression profiles (similarly normalized) from 10 human normal ovarian surface epithelial (OSE) cell brushings available in the ArrayExpress database (www.ebi.ac.uk/arrayexpress/), accession number E-GEOD-18520.
Experiment types
transcription profiling by array, array platform variation, co-expression, disease state
Contact
Citation
Small cell ovarian carcinoma: genomic stability and responsiveness to therapeutics.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3872.idf.txt
Sample and data relationshipE-MEXP-3872.sdrf.txt
Raw data (1)E-MEXP-3872.raw.1.zip
Processed data (1)E-MEXP-3872.processed.1.zip
Array designA-AFFY-44.adf.txt
Links