E-MEXP-3852 - Transcription profiling by array of Pleuronichthys verticalis (Hornyhead turbot) captured from wastewater treatment plant discharge locations to investigate endocrine disruption
Released on 10 December 2013, last updated on 3 May 2014
Hornyhead turbot (Pleuronichthys verticalis) captured near sewage outfalls are used as sentinel fish for monitoring exposure to industrial and agricultural chemicals of ~20 million people living in coastal southern California. Although analyses of hormones in blood and organ morphology and histology in fish are useful for assessing exposure, there is a need for quantitative and sensitive molecular measurements, as many contaminants produce subtle effects. A novel multispecies microarray and qRT-PCR were used to investigate endocrine disruption in turbot captured near sewage outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g. estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with phenotypic end points.
transcription profiling by array, co-expression, reference design, replicate design, stimulus or stress design
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. Baker ME, Vidal-Dorsch DE, Ribecco C, Sprague LJ, Angert M, Lekmine N, Ludka C, Martella A, Ricciardelli E, Bay SM, Gully JR, Kelley KM, Schlenk D, Carnevali O, Sášik R, Hardiman G . PloS one 8(9):e75553 (2013), Europe PMC 24086568