E-MEXP-3850 - Transcription profiling by array of venous blood samples from paediatric patients with clinical meningococcal sepsis over a 48-hour time-course
Released on 3 April 2013, last updated on 3 May 2014
The temporal evolution of sepsis was monitored by transcriptional profiling of five critically ill children with meningococcal sepsis and sepsis-induced multiple organ failure. Blood was sampled at 6 time points during the first 48 hours of their admission to pediatric intensive care, where the children received standard clinical treatment including organ support and antimicrobial therapy. Striking transcript instability was observed over the 48 hours, with increasing numbers of regulated genes over time. Most notably, proposed biomarkers for sepsis risk stratification also showed expression instability, with varied expression levels over 48 hours. This study demonstrates the extent of the complexity of temporal changes in gene expression that occur during the evolution of sepsis-induced multiple organ failure. Importantly, stratification tools that propose expression of biomarkers must take into account the temporal changes, over the use of single snapshots that may be less informative.
transcription profiling by array, co-expression, in vivo, time series
Transcriptional instability during evolving sepsis may limit biomarker based risk stratification. Kwan A, Hubank M, Rashid A, Klein N, Peters MJ. :e60501 (2013), Europe PMC 23544148
Oxidative phosphorylation gene expression falls at onset and throughout the development of meningococcal sepsis-induced multi-organ failure in children. Raman S, Klein N, Kwan A, Hubank M, Rahman S, Rashid A, Peters MJ. :1489-1490 (2015), Europe PMC 25920543
Transcriptional Instability During Evolving Sepsis May Limit Biomarker Based Risk Stratification. Kwan A, Hubank M, Rashid A, Klein N, Peters MJ. PLoS One 8(3):e60501 (2013), Europe PMC 23544148