E-MEXP-3833 - Transcription profiling by array of mouse embryo lungs to investigate how endogenous retinoic acid (RA) and glucocorticoids (GCs) regulate gene expression during development
Last updated on 18 February 2013, released on 12 January 2014
Mammalian lung development during the saccular and alveolar stages is dependent upon antagonistic molecular signalling by endogenous retinoic acid (RA) and glucocorticoids (GCs) which regulate gene expression via the retinoic acid receptor (RAR) family and the glucocorticoid receptor (GR), respectively. The genomic mechanism of this antagonism was investigated with in vitro distal lung explant cultures from E18.5 GR-null (GR-/-) mice treated with all-trans-RA (atRA) for 2h . Whole mouse genome microarray analysis from lung explant tissue identified a small number of gene targets which were not only significantly induced by atRA in the wildtype lung, but also significantly stimulated to levels greater than atRA-treated wildtype lungs in GR-/- lungs.
transcription profiling by array, all pairs, co-expression, individual genetic characteristics