E-MEXP-3805 - Transcription profiling by array of human monocyte-derived macrophages infected with Mycobacterium tuberculosis (H37Rv strain)

Status
Released on 1 June 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (6)
Description
Human monocyte-derived macrophages were infected with Mycobacterium tuberculosis (H37Rv strain) at a multiplicity of infection 1:1. After 2h of incubation, cells were washed to remove extracellular bacteria and were incubated in fresh medium for a further 18h in the presence or not of 100microM ATP. Total RNA was then extracted and gene expression was determined using HuGene 1.0 ST microarrays (Affymetrix, Inc.,Santa Clara, CA).
Experiment types
transcription profiling by array, co-expression, compound treatment, disease state design, in vitro
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MEXP-3805.idf.txt
Sample and data relationshipE-MEXP-3805.sdrf.txt
Raw data (1)E-MEXP-3805.raw.1.zip
Array designA-AFFY-141.adf.txt
Links