nucleic acid labeling protocl
WT-Ovation RNA Amplification System (NuGEN Technologies Inc., San Carlos, CA, USA). The reaction products were purified with a QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany).
(Parameters: Amplification = PCR, Mass unit = Micro gram)
blood was drawn into the PAXgeneTM Blood RNA tubes (PreAnalytix, Hilden, Germany) by standard phlebotomy procedures, the samples were inverted ten times, maintained at room temperature for 2 hours, frozen at -20? overnight and then moved to -80? for storage until RNA extraction.
nucleic acid extraction protocol
Frozen PAXgeneTM Blood RNA tubes were thawed at room temperature for 3 hours, followed by total RNA extraction with the PAXgeneTM Blood RNA kit according to the manufacturer's instruction.
(Parameters: Extracted product = total_RNA, Amplification = none)