7 protocols
AccessionType
array scanning and feature extraction protocol
Scanning of Affymetrix GeneChip microarrays was carried out according to the manufacturer’s standard protocol
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
bioassay_data_transformation
Title: Affymetrix CHP Analysis (ExpressionCall). Description:
hybridization
Title: Affymetrix EukGE-WS2 Hybridization. Description:
nucleic_acid_extraction
Total RNA was extracted from 50 to 100mg of tissues using Trizol. RNA samples were then purified using RNeasy mini kit.
(Parameters: Extracted product = total_RNA, Amplification = none)
specified_biomaterial_action
Two rosette leaves were treated with heat (40C), high light (1000uE) or wounding and meristems were sampled at 15 and 45min following stress treatment. Samples were also collected in parallel from plants that were exposed to control treatments corresponding to each stress.
grow
Arabidopsis Col-0 plants were grown on peat pellet for 3 weeks under controlled conditions (21C, 16h light).
(Parameters: time unit = seconds, temperature unit = C)