E-MEXP-3749 - New target genes of MITF-induced microRNA-211 contribute to melanoma cell invasion
Last updated on 1 August 2013, released on 1 August 2013
IGR39 cells, seeded at a density of 6x104 cells/well in 12-well culture plates, were transfected with 5 nM of miR-211 mimic or negative control (NCM) as described in the main text. Samples for total RNA extraction were collected 48h after transfection. RNA quality was assessed using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent, Paolo Alto, USA). Gene expression profiling experiments using GeneChip® Human Gene 2.0 ST arrays and miRNA profiling using GeneChip® miRNA arrays (Affymetrix, Santa Clara, CA, USA) were performed on two independent samples at the CRP-Santé Microarray Unit (Luxembourg) as described before (Reinsbach et al., 2012) and according to standard protocols. Standard pipeline from the Partek® Genomics SuiteTM (Partek GS) software was used for analysis of data files. Lists of genes were generated by pair-wise comparison of expression data sets (negative control vs. mimic treated samples at 48h).
transcription profiling by array, cellular modification, co-expression, is expressed, replicate
New target genes of MITF-induced microRNA-211 contribute to melanoma cell invasion. Christiane Margue§, Demetra Philippidou§, Susanne E. Reinsbach, Martina Schmitt, Iris Behrmann and Stephanie Kreis. PloS One (2013)