E-MEXP-3748 - Transcription profiling by array of mouse embryonic stem cells overexpressing let-7, miR-99a or both during the early stages of cardiac differentiation

Status
Released on 1 July 2013, last updated on 20 October 2015
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (5)
Description
To investigate the function of miR99a/let-7c miRNAs during cardiomyogenesis, we decided to perturb their expression using transgenic mES cell lines the corresponding precursors (pre-miRNA) of the two miRNAs. We used the ROSA-TET system (EB3 tet off), in which the transgene is inserted in Rosa26 locus by Cre recombinase. We generated three different clones both miRNAs let-7c/miR-99a and those only one miRNA, namely the let-7cdeletedmiR-99a and the miR-99adeletedlet-7c.
Experiment types
transcription profiling by array, co-expression, compound treatment design, genetic modification design, in vitro
Contact
Citation
Cardiomyogenesis is controlled by the miR-99a/let-7c cluster and epigenetic modifications. Coppola A, Romito A, Borel C, Gehrig C, Gagnebin M, Falconnet E, Izzo A, Altucci L, Banfi S, Antonarakis SE, Minchiotti G, Cobellis G. , Europe PMC 24365598
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3748.idf.txt
Sample and data relationshipE-MEXP-3748.sdrf.txt
Raw data (1)E-MEXP-3748.raw.1.zip
Array designA-AFFY-45.adf.txt
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