E-MEXP-3748 - Transcription profiling by array of mouse embryonic stem cells overexpressing let-7, miR-99a or both during the early stages of cardiac differentiation
Released on 1 July 2013, last updated on 20 October 2015
To investigate the function of miR99a/let-7c miRNAs during cardiomyogenesis, we decided to perturb their expression using transgenic mES cell lines the corresponding precursors (pre-miRNA) of the two miRNAs. We used the ROSA-TET system (EB3 tet off), in which the transgene is inserted in Rosa26 locus by Cre recombinase. We generated three different clones both miRNAs let-7c/miR-99a and those only one miRNA, namely the let-7cdeletedmiR-99a and the miR-99adeletedlet-7c.
transcription profiling by array, co-expression, compound treatment design, genetic modification design, in vitro
Cardiomyogenesis is controlled by the miR-99a/let-7c cluster and epigenetic modifications. Coppola A, Romito A, Borel C, Gehrig C, Gagnebin M, Falconnet E, Izzo A, Altucci L, Banfi S, Antonarakis SE, Minchiotti G, Cobellis G. , Europe PMC 24365598