E-MEXP-3748 - Transcription profiling by array of mouse embryonic stem cells to investigate the function of miR99a/let-7c miRNAs during cardiomyogenesis
Released on 1 July 2013, last updated on 3 May 2014
To investigate the function of miR99a/let-7c miRNAs during cardiomyogenesis, we decided to perturb their expression using transgenic mES cell lines the corresponding precursors (pre-miRNA) of the two miRNAs. We used the ROSA-TET system (EB3 tet off), in which the transgene is inserted in Rosa26 locus by Cre recombinase. We generated three different clones both miRNAs let-7c/miR-99a and those only one miRNA, namely the let-7cdeletedmiR-99a and the miR-99adeletedlet-7c.
transcription profiling by array, co-expression, compound treatment design, genetic modification design, in vitro