4 protocols
AccessionNameType
P-MTAB-28611
specified_biomaterial_action
Cells were serum starved (0% serum) for 4 hours then treated with 10ng/ml IFN-alpha2b (Promokine, Germany) or vehicle control in EGM-MV2 5%FCS for 6 hours.
P-MTAB-28608
grow
Human Arotic Endothelial Cells (HAoECs) from a single donor (Promocell, Germany) were cultured in EGM-MV2 5% FCS in uncoated plastic ware in standard culture conditions (5% CO2, humidified environment). Cells used at passages 6 and 7. (Parameters: time unit = seconds, min temperature = 37, temperature unit = C, media = EGM-MV2)
P-MTAB-28610
bioassay_data_transformation
Exons of the Core Meta-Probeset were used. Quantile Normalisation and RMA background correction was performed. Data analysis performed with Partek Genomics Suite, version 6.3, Copyright 2007, Partek Inc., St. Charles, MO, USA.
P-MTAB-28609
nucleic_acid_extraction
Cells were lysed in 1ml TRI Reagent (Sigma-Aldrich) followed by RNA extraction according to the manufacturer’s instructions. To remove any genomic DNA, RNA was treated with DNase I (Ambion) at 37C for 30 mins and the reaction stopped by addition of Phenol:Chloroform:IAA. Following centrifugation at 12,000xg for 5 mins at 4C, the aqueous phase was removed and RNA precipitated during a 30 minute incubation on ice with 1/10 ammonium acetate (Ambion) and 3x 100% ethanol. Centrifugation at 12,000xg for 20 mins at 4C produced an RNA pellet which was washed in 75% ethanol, centrifuged again for 5 minutes, before air-drying and dissolving in tris-EDTA buffer (Ambion). (Parameters: Extracted product = total_RNA, Amplification = none)