5 protocols
AccessionType
array scanning and feature extraction protocol
Scanning of Affymetrix GeneChip microarrays was carried out according to the manufacturer’s standard protocol
hybridization protocol
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic_acid_extraction
Sorted cells were lysed were lysed in RLT buffer (Qiagen) and further processed on Qiagen Rneasy Micro columns including DNAse treatment (Parameters: Extracted product = total_RNA, Amplification = none)
specified_biomaterial_action
GVHD was induced in recipients, using a haploidentical P into F1 murine transplantation model. Some recipients were injected i.p. peritransplantally with 5mg/kg/day Fgf7 for 7 days. Control group received HBSS. Recipient's thymi were dissected, digested with Collagenase/Dispase/Dnase and thymic epithelial cells were sorted by FACS.