E-MEXP-3745 - Transcription profiling by array of murine thymus medulla in the absence and presence of acute graft-versus-host disease to investigate gene expression profile of medullary thymic epithelial cells with high surface densitiy of MHC class II
Last updated on 26 June 2013, released on 27 June 2013
To investigate gene expression profile of medullary thymic epithelial cells with high surface densitiy of MHC class II (mTEChigh), a murine parent into F1 hematopoietic stem cell transplantation model was administered. As recipient mice (B6xDBA/2)F1 (B6D2F1) were used that received either a syngeneic transplant from a (B6xDBA/2)F1 mouse or an allogeneic transplant from a B6 mouse, which leads to acute graft-versus-host disease (aGVHD). mTEChigh (CD45-EpCAM+Ly51-UEA1+MHCIIhigh) are sorted by FACS.
transcription profiling by array, co-expression, compound treatment design, disease state, in vivo
Epithelial cytoprotection sustains ectopic expression of tissue-restricted antigens in the thymus during murine acute GVHD. Dertschnig S, Nusspaumer G, Ivanek R, Hauri-Hohl MM, Hollander GA, Krenger W . Blood (2013)